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Objective To investigate the changes in serum hyaluronic acid level at different periods of experimental piggyback liver transplantation and its significance. Methods Fifteen pairs of healthy pigs of both sexes weighing (28.3 + 5.0)kg undergoing liver transplantation were studied. The donor pigs were slightly smaller than the recipient pigs. The recipient pigs were premedicated with intramuscular ketamine 8mg kg-1 and atropine 0.02mg-kg-1 .Anesthesia was induced with propofol 2mg-kg-1 , fentanyl 0.002mg-kg-1 and vecuronium 0.1mg-kg-1 iv. After tracheal intubation the animals were mechanically ventilated. CVP line was placed via internal jugular vein. Carotid artery was cannulated for continuous BP monitoring. Anesthesia was maintained with iv propofol, fentanyl and vecuronium. Circulatory stability was maintained by infusion of crystalloid, colloid, plasma and whole blood of pig. Blood samples were taken from peripheral vein before operation (T0), pre-anhepatic phase (T1), anhepatic phase (T2) and neohepatic phase (T3) for determination of serum hyaluronic acid concentration by radioimmunoassay. At the same time liver function tests, ALT, AST, y-GT were also examined. Results The average time of liver transplantation was (343+74) min. SBP decreased by (23.51+5.87 ) mm Hg and DBF by (11.35+7.81) mm Hg after induction of anesthesia. Serurn hyaluronic acid level was 267ug-L-1 before operation (T0) and rose to 1743ug-L-1 at T1 , and 9530ug-L-1 at T2 (P
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Objective To evaluate the effects of propofol and isoflurane on the change in periopreative serum IL 6,IL 8,IL 10 and the balance between pro and anti inflammatory cytokines.Methods Twenty five ASAⅡ Ⅲ patients scheduled for esophagus cancer surgery were randomly allocated to one of the two groups: isoflurane group(group I) and propoful group(group P). All patients were premedicated with oral diazepam 5mg and intramuscular atropine 0.3mg . In group P anesthesia was induced with propofol 1 2 mg?kg -1 ,fentanyl 2 ?g?kg -1 ,midazolam 2 3 mg and vecuronium 0.1mg?kg -1 and maintained with propofol 5 10 mg?kg -1 ?h -1 , inhalation of nirous oxide (N 2O:O 2=50%:50%) and intermittent of boluses of vecuronium. In group I anesthesia was induced with 3% 4% isoflurane ,fentanyl 2?g?kg -1 ,midazolam 2 3mg, vecuronium 0.1mg?kg -1 and maintained with inhalation of 50% N 2O and isoflurane (ended tidal isoflurane was maintained at 0.6%) and intermittent boluses of vecuronium. During operation BP and HR were maintained with at ?20% of pre anesthesia level. After operation all patients received PCEA with 0.15% bupivacaine and fentanyl 2?g?ml -1 . Blood samples were taken from internal jugular vein before anesthesia (T 0), at skin incision(T 1), 2h after thoracotomy (T 2), 60min after lung were inflated(T 3),1,4 and 24h after operation(T 4,5,6 ) for determination of serum IL 6,IL 8 and IL 10 concentrations. Results All patients showed significant increases in IL 6, IL 8 and IL 10 levels during thoracotomy (P
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Objective To compare the effect of midazolam and propofol on cytokine balance in patients undergoing cardiac valve replacement under CPB. Methods Twenty NYHA class Ⅱ - Ⅲ patients with rheumatic heart disease scheduled for elective cardiac valve replacement under CPB were randomly divided into 2 groups: midazolam group (M, n = 10) and propofol group (P, n = 10) . Patients with preoperative hepatic and renal dysfunction, coagulation defect and diabetes, primary hypertension and coronary artery disease were excluded. The patients were premedicated intramuscular morphine 0.1 mg?kg-1 and oral diazepam 0.1 mg?kg-1. Anesthesia was induced with midazolam 0.06-0.1 mg?kg-1 (group M) or propofol 1.0-1.5 mg?kg-1 (group P) and scopolamine 0.6 mg, fentanyl 4-10 ug?kg-1, vecuronium 0.1 mg?kg-1 and lidocaine 1.5 mg?kg-1, and maintained with intermittent iv boluses of midazolam 0.04-0.1 mg? kg-1 (group M) or propofol intravenous infusion at a rate of 3-5 mg?kg-1?h-1 supplemented with intermittent boluses of fentanyl and vecuronium. PETCO2 was maintained at 30-40 mm Hg during mechanical ventilation. BP, HR, ECG, CVP, PETCO2, SpO2 , nasal and rectal temperature were continuously monitored during operation. Blood samples were taken from artery before anesthesia (T1), at vena cava catheterization (T2), 30min after aortic cross-clamping (T3) and 10 min (T4), 1 h (T5), 2 h(T6), 4 h(T7) and 24 h(T8) after declamping of aorta for determination of Hct, IL-1, IL-8 and IL-10 by using ELISA. Results Plasma concentrations of IL-6, IL-8 and IL-10 were significantly increased at T4-7 as compared with the baseline value before anesthesia (T1) in both group. Plasma concentration of IL-8 in group P was significantly lower than that in group M at T7 ( P
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OBJECTIVE:To gain a clear idea about the situation of marking the period of validity on the wrapper and specifications of patent Chinese materia medica(PCMM) in China METHODS:To investigate the period of validity marking on wrapper and internal packing of 369 commonly-used PCMM in our hospital RESULTS:The PCMM with period of validity marking accounted for 65 9% of total PCMM(usually 1 5～3 years) and those without period of validity accounted for 34 1% CONCLUSION: The absence of period of validity marking is very common,we should pay attention to it